Friday, March 11, 2011

The use of RAPD in assessing genetic variability in Andrographis paniculata Nees

Abstract: 
RAPD analysis was done to determine intraspecific variability in Andrographis paniculata, a popular antipyretic and hepatoprotective drug used in traditional medicine in India. The accessions collected from parts of India and southeast Asia on molecular analysis revealed moderate variation within the species. Similarity measurement using UPGMA followed by cluster analysis resulted in 5 major groups based on geographical distribution that generally reflected expected trends between the genotypes. There were also important exceptions like AP48, an accession from Thailand showing close resemblance to AP38 collected from Tamil Nadu and AP29 from Assam significantly diverse from the rest of the native genotypes. The results indicated that RAPD could be effectively used for genetic diversity analysis in wild species of prospective value as it is reliable, rapid and superior to those based on pedigree information.

Published in:
Current Science (Bangalore) 76: 833-835

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