Thursday, April 8, 2021

Impact of potential stimulants on asiaticoside and madecassoside levels and expression of triterpenoid-related genes in axenic shoot cultures of Centella asiatica (L.) Urb

Gouri Priya Ranjith, S. Jisha, Achutan Sudarsanan Hemanthakumar, Chinthu V. Saji, Rajesh A. Shenoi, Kallevettankuzhy Krishnannair Sabu

Phytochemistry, Volume 186, 2021, 112735

The triterpenoid saponinsasiaticoside and madecassoside from Centella asiatica (L.) Urb. are known to have a wide range of applications in pharmaceutical and cosmetic industries. The effect of addition of Potential Metabolite Stimulants (PMSs) - casein acid hydrolysate, meat peptone, salicylic acid, copper sulphate, and silver nitrate, on the concentrations of these saponins and transcript levels of associated genes encoding important biosynthetic enzymes, was assessed in axenic shoot cultures of C. asiatica. Among the stimulants, silver nitrate induced asiaticoside content approximately 6-fold increase in madecassoside levels, after three weeks post-treatment with a decrease in biomass compared to its control. Gene expression analysis of essential genes involved in triterpenoid synthesis such as β-amyrin synthase showed an upregulation of approximately 50-fold at the third week of silver nitrate treatment compared to control. These findings suggest that silver nitrate can act as a metabolite stimulant, to enhance the formation of triterpenoids in axenic shoot culture of C. asiatica, which could be utilized in studying the regulation of terpenoid biosynthesis and biotechnological application for the increased production of these bioactive molecules.

https://doi.org/10.1016/j.phytochem.2021.112735

Thursday, January 28, 2021

Phytochemical profiling and antioxidant activities of different parts of Artocarpus heterophyllus Lam. (Moraceae): A review on current status of knowledge

Artocarpus heterophyllus Lam. (Jackfruit tree) is one of the most ancient fruits indigenous to Western Ghats of India. They are large edible summer fruits and are good source of nutritional and antioxidant components which hold their potential for nutraceutical development. The present review attempts to document reports on the chemical and pharmacological investigations carried out with jackfruit. Research shows that the fruit contains a huge amount of phytochemicals such as phenolics, flavonoids, terpenoids, steroids, glycosides, saponins, alkaloids, and tannins which exhibit antioxidant properties. Diversity of secondary metabolites present in the jackfruit shows that it is a functionally, nutritionally, and medicinally important fruit. Detailed studies are essential for awareness among people for the proper use of jackfruit as a functional food and utilize the medicinal uses of jackfruit.

Sreeja Devi P S, Neethu S Kumar, K K Sabu. 2021. Phytochemical profiling and antioxidant activities of different parts of Artocarpus heterophyllus Lam. (Moraceae): A review on current status of knowledge. Future Journal of Pharmaceutical Sciences 7, Article number: 30. Published: 28 January 2021. https://doi.org/10.1186/s43094-021-00178-7

Friday, January 8, 2021

Genetic resources of Asian Palmyrah palm (Borassus flabellifer L.): A comprehensive review on diversity, characterization and utilization

Asian palmyrah palm (Borassus flabellifer L.) is a multipurpose, economically important tree widely distributed in the Indian subcontinent and Southeast Asia. The multifaceted uses (~800 uses) of the tree as food, wood and medicine make it a viable industrial crop. However, the dioecious nature of the tree coupled with long juvenile phase (12–20 years to initiate flowering), long stature and considerable variations in the productivity of male and female trees necessitates the application of new biotechnological tools for crop improvement. This review provides comprehensive information on the extent of genetic diversity of the crop, problems associated with palmyrah cultivation along with its multifaceted application and research gaps to be addressed for the effective utilization and conservation of palmyrah palm genetic resources.

Siju S. and K. K. Sabu. 2021. Genetic resources of Asian Palmyrah palm (Borassus flabellifer L.): A comprehensive review on diversity, characterization and utilization. Plant Genetic Resources. Published online: 08 January 2021. https://doi.org/10.1017/S1479262120000477

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